rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
4
|
pubmed:dateCreated |
1989-12-21
|
pubmed:abstractText |
1. Elastin was isolated from the bulbus arteriosus of a salmonid fish. Monoclonal and polyclonal antibodies, elicited against a CNBr digest of this protein, immunoprecipitated a polypeptide of Mr 43,000 from fish cell culture medium. 2. Cell-free translation of salmon poly A+ RNA produced a protein of approximately 43 kD that was immunoprecipitated with anti-elastin antibodies. The corresponding mRNA had an approximate Mr of 2 kb. 3. Despite similarities in amino acid composition, the differences in Mr between mammalian and salmon mRNA and protein suggest a divergence of fish and higher vertebrate elastins from an earlier ancestral gene.
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pubmed:grant |
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:issn |
0305-0491
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
93
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
835-45
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:2805642-Amino Acids,
pubmed-meshheading:2805642-Animals,
pubmed-meshheading:2805642-Antibodies,
pubmed-meshheading:2805642-Antibodies, Monoclonal,
pubmed-meshheading:2805642-Blotting, Western,
pubmed-meshheading:2805642-Cell-Free System,
pubmed-meshheading:2805642-Elastin,
pubmed-meshheading:2805642-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:2805642-Immune Sera,
pubmed-meshheading:2805642-Protein Biosynthesis,
pubmed-meshheading:2805642-RNA, Messenger,
pubmed-meshheading:2805642-Salmon,
pubmed-meshheading:2805642-Tropoelastin
|
pubmed:year |
1989
|
pubmed:articleTitle |
Characterization of elastin protein and mRNA from salmonid fish (Oncorhynchus kisutch).
|
pubmed:affiliation |
Department of Biological Structure, University of Washington, Seattle 98195.
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|