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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1989-12-5
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pubmed:abstractText |
1. It has been suggested that low levels of serum caeruloplasmin in Wilson's disease result from the failure to switch from a fetal to an adult mode of caeruloplasmin gene expression. To investigate postnatal expression of the caeruloplasmin gene, steady-state levels of caeruloplasmin messenger RNA in adult and neonatal rat liver were measured. 2. Copper parameters observed in neonatal rats were similar to those seen in Wilson's disease: hepatic copper concentration was significantly elevated (neonatal 164 +/- 35 micrograms/g, adults 50 +/- 8 micrograms/g, P less than .001) and serum copper and caeruloplasmin levels were low (neonatal 0.5 +/- 0.1 microgram/ml, adults 1.3 +/- 0.2 microgram/ml, P less than .001; neonatal 0.20 +/- 0.04 arbitrary units, adults 0.69 +/- 0.16 arbitrary units, P less than .001), respectively. 3. Caeruloplasmin messenger RNA levels were analysed by Northern and dot blotting using a 32P-labelled caeruloplasmin complementary DNA probe. A caeruloplasmin messenger RNA of approximately 4.4 kilobases was detected in both adult and neonatal rat liver, with no significant difference observed in steady-state levels. 4. A step subsequent to caeruloplasmin gene transcription must therefore be impaired in neonatal rats.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0143-5221
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
77
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
259-63
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2805592-Animals,
pubmed-meshheading:2805592-Animals, Newborn,
pubmed-meshheading:2805592-Blotting, Northern,
pubmed-meshheading:2805592-Ceruloplasmin,
pubmed-meshheading:2805592-Copper,
pubmed-meshheading:2805592-Genes,
pubmed-meshheading:2805592-Liver,
pubmed-meshheading:2805592-Male,
pubmed-meshheading:2805592-RNA, Messenger,
pubmed-meshheading:2805592-Rats,
pubmed-meshheading:2805592-Rats, Inbred Strains,
pubmed-meshheading:2805592-Serum Albumin
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pubmed:year |
1989
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pubmed:articleTitle |
Expression of the caeruloplasmin gene in the adult and neonatal rat liver.
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pubmed:affiliation |
Department of Child Health, University of Leicester, U.K.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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