Linked Life Data

2805073

Source:http://linkedlifedata.com/resource/pubmed/id/2805073

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1989-12-7
pubmed:abstractText
Human alloreactive T-cell clones derived from cells invading a rejected kidney allograft were found to be capable of proliferating upon specific antigenic stimulation in short term assays and in the absence of exogenous IL-2. In most culture supernatants there was no evidence of any activity capable of triggering proliferation of the murine IL-2-sensitive CTL-L2 line. The ability of these clones to produce IL-2 was investigated under several experimental conditions. A combination of phorbol ester plus calcium ionophore was very efficient in all clones tested (17/17) in inducing transcription of the IL-2 gene. Conversely, when cell activation took place through the CD3/Ti complex, we regularly failed to detect hu-IL-2 transcripts even when the number of stimulator cells was increased. However, a blockade of IL-2-R with a monoclonal antibody inhibiting ligand receptor interaction while decreasing proliferation induced by the antigen in dose-dependent fashion permitted the presence of IL-2 to be demonstrated in supernatants. These results strongly suggest that IL-2-driven proliferation is essential in this model and may represent a major, although not exclusive, pathway leading to proliferation of these clones.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0008-8749
pubmed:author
pubmed:issnType
Print
pubmed:volume
124
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
95-106
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Proliferation dependence and production of IL-2 in human alloreactive T-cell clones.
pubmed:affiliation
INSERM U.211. Laboratorie d'Immunologie Clinique, Faculté de Médecine, Nantes, France.
pubmed:publicationType
Journal Article