Linked Life Data

2804074

Source:http://linkedlifedata.com/resource/pubmed/id/2804074

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1989-12-13
pubmed:abstractText
A fluorescent acyl derivative of pyrenemethanol, pyrenemethyl laurate, was synthesized and used for the determination of several lipases by a continuous kinetic assay. The influence of the physical parameters of the substrate (pyrenemethyl laurate) and its hydrolysis product (pyrenemethanol), on the fluorescence emission was studied. The hydrolysis of pyrenemethyl laurate could be monitored directly in a spectrofluorometer because of the very high monomeric emission of pyrenemethanol at about 375 nm, whereas an aqueous dispersion of pyrenemethyl laurate emitted at 475 nm ('excimeric'). Pyrenemethyl laurate was hydrolyzed by gastric lipase, cellular lipases of haemopoietic cells, and the bacterial lipase of Rhizopus arrhizus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
1006
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
84-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Pyrenemethyl laurate, a new fluorescent substrate for continuous kinetic determination of lipase activity.
pubmed:affiliation
Laboratoire de Biochimie, Faculté de Médecine, Toulouse, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't