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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1989-12-1
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pubmed:abstractText |
The effects of a novel anti-cancer drug, YM 534 on human promyelocytic leukemia line HL-60 cells were investigated. The growth of the cells was completely inhibited with an IC50 of 2.5 X 10(-6) M. The incorporation of 3H-thymidine or 3H-uridine into acid insoluble fraction of the cells was completely inhibited at a 10(-4) M concentration of the drug. However, that of 3H-leucine was suppressed by only 32% at this drug concentration. The single strand scission of DNA of the cells as revealed by alkali sucrose density gradient centrifugation was induced by YM 534 at a 10(-4)M concentration for 60 min. When PM2 DNA in a cell-free system was examined in the presence or absence of reducing agent, no damage to DNA was observed at a 10(-4)M YM 534. The processing of preribosomal to ribosomal RNA of HL-60 cells was retarded at a 10(-5) M level of the drug.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0266-9536
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
137-44
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:2803462-Antineoplastic Agents,
pubmed-meshheading:2803462-Cell Line,
pubmed-meshheading:2803462-DNA, Neoplasm,
pubmed-meshheading:2803462-DNA Damage,
pubmed-meshheading:2803462-Dose-Response Relationship, Drug,
pubmed-meshheading:2803462-Drug Evaluation, Preclinical,
pubmed-meshheading:2803462-Humans,
pubmed-meshheading:2803462-Imidazoles,
pubmed-meshheading:2803462-Leukemia, Promyelocytic, Acute,
pubmed-meshheading:2803462-RNA, Neoplasm,
pubmed-meshheading:2803462-RNA, Ribosomal,
pubmed-meshheading:2803462-RNA Precursors,
pubmed-meshheading:2803462-RNA Processing, Post-Transcriptional,
pubmed-meshheading:2803462-Tumor Cells, Cultured
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pubmed:year |
1989
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pubmed:articleTitle |
Mechanism of action of the synthetic imidazole compound YM 534 on human promyelocytic leukemia line HL-60 cells.
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pubmed:affiliation |
Department of Clinical Cancer Chemotherapy, Tohoku University, Sendai, Japan.
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pubmed:publicationType |
Journal Article
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