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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1989-11-20
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pubmed:abstractText |
Recently, the two Mr forms of caldesmon (Mr's in the range of 120-150kDa and 70-80kDa as judged by SDS-PAGE) have been identified. h-Caldesman (high Mr 120-150kDa caldesmon) is predominantly expressed in smooth muscles, and l-caldesmon (low Mr 70-80kDa caldesmon) in non-muscle cells. In this paper, we report the nucleotide sequence of chick embryo gizzard h-caldesmon cDNA and its translation into amino acid sequence. This sequence predicts a protein of 771 amino acids with a Mr of 88,743. The central portion of this sequence is composed of a 10-fold repeat of conserved amino acid sequence containing 13-15 amino acids. Further, a recombinant protein produced in Escherichia coli containing the full-length h-caldesmon cDNA has been characterized. Although the Mr of h-caldesmon predicted from amino acid sequence is 88,743, native and recombinant proteins show the same mol. wt. with 150kDa as measured by SDS-PAGE. This discrepancy may be due to the acidic amino acid-rich sequences at the N-terminal and central portions. A recombinant protein produced in E. coli possesses calmodulin-, F-actin- and tropomyosin-binding abilities in common with the native h-caldesmon.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
164
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
503-11
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2803315-Amino Acid Sequence,
pubmed-meshheading:2803315-Animals,
pubmed-meshheading:2803315-Base Sequence,
pubmed-meshheading:2803315-Calmodulin-Binding Proteins,
pubmed-meshheading:2803315-Chickens,
pubmed-meshheading:2803315-Cloning, Molecular,
pubmed-meshheading:2803315-DNA,
pubmed-meshheading:2803315-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2803315-Molecular Sequence Data,
pubmed-meshheading:2803315-Nucleic Acid Conformation,
pubmed-meshheading:2803315-Plasmids,
pubmed-meshheading:2803315-Recombinant Proteins,
pubmed-meshheading:2803315-Repetitive Sequences, Nucleic Acid
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pubmed:year |
1989
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pubmed:articleTitle |
Primary structure and functional expression of h-caldesmon complementary DNA.
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pubmed:affiliation |
Department of Neurochemistry and Neurophamacology, Osaka University Medical School, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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