Linked Life Data

2802999

Source:http://linkedlifedata.com/resource/pubmed/id/2802999

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1989-11-16
pubmed:abstractText
DNA was synthesized complementary to the RNA genome of potato virus Y (PVY; strain GO 16) and cloned into lambda vectors. The size of PVY-specific Eco RI-restricted cDNA ranged from 0.3 to approximately 22kb. Two of the cDNA clones each of which contained some 4kb of cDNA sequence starting from the 3'-polyadenylated terminus were characterized by sequence analysis. Presence of a single open reading frame suggests that PVY-specific proteins are synthesized as a polyprotein precursor. As with other sequenced potyvirus RNAs the gene for the PVY capsid protein CP is located next to the 3'-untranslated region followed by the genes for the putative RNA polymerase (nuclear inclusion protein NIb) and the virus-specific protease (nuclear inclusion protein NIa). The 3'-trailing sequence of the PVY strains cloned is highly homologous to the corresponding region of pepper mottle virus (PeMV) and suggests that PeMV is not a distinct member of the potyvirus group, but another strain of PVY.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0304-8608
pubmed:author
pubmed:issnType
Print
pubmed:volume
107
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
123-34
pubmed:dateRevised
2007-10-11
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Cloning of the potato virus Y genes encoding the capsid protein CP and the nuclear inclusion protein NIb.
pubmed:affiliation
Max-Planck-Institut für Züchtungsforschung, Köln, Federal Republic of Germany.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't