Linked Life Data

2792562

Source:http://linkedlifedata.com/resource/pubmed/id/2792562

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1989-11-22
pubmed:abstractText
Expressing foreign proteins in heterologous eukaryotic cells has been a powerful tool for analyzing protein structure and function. The inducible mouse metallothionein-I promoter has been particularly useful for expression studies. However, the levels of expression achieved with this promoter in heterologous eukaryotic expression systems have not equaled those observed in vivo for the metallothionein-I gene. We have constructed expression plasmids placing either the gene for chloramphenicol acetyltransferase (CAT) or the cDNA for human neuropeptide Y (NPY) under control of the mouse metallothionein-I promoter. These two expression vectors were used to transfect mouse anterior pituitary tumor cells, from which stable transformants were isolated. The resulting cell lines, Mt.NPY1a and Mt.CAT, were used to maximize functional product expression from the metallothionein-I promoter. In both cell lines, a 35-fold induction of mRNA accumulation, peptide synthesis, or CAT activity was observed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0303-7207
pubmed:author
pubmed:issnType
Print
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
205-12
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Metallothionein-I promoter-directed expression of foreign proteins in a mouse pituitary corticotrope tumor cell line.
pubmed:affiliation
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.