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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1989-11-9
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pubmed:databankReference |
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pubmed:abstractText |
A cDNA library from Aspergillus niger strain NRRL-3 enriched in sequences glucose oxidase was constructed. An 800 bp cDNA clone isolated from this library was used to screen 12,000 recombinant phages from an EMBL3 genomic library. A 15 kbp DNA segment isolated from this library contained the 1815 bp structural gene for glucose oxidase as well as a short 5'- and a longer 3'-noncoding region. The deduced protein sequence was verified by partial peptide sequencing.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
255
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
63-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2792372-Amino Acid Sequence,
pubmed-meshheading:2792372-Aspergillus niger,
pubmed-meshheading:2792372-Base Sequence,
pubmed-meshheading:2792372-Cloning, Molecular,
pubmed-meshheading:2792372-DNA,
pubmed-meshheading:2792372-Genes, Fungal,
pubmed-meshheading:2792372-Glucose Oxidase,
pubmed-meshheading:2792372-Molecular Sequence Data,
pubmed-meshheading:2792372-Oligonucleotides,
pubmed-meshheading:2792372-RNA, Messenger,
pubmed-meshheading:2792372-Recombinant Proteins
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pubmed:year |
1989
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pubmed:articleTitle |
Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL-3.
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pubmed:affiliation |
Institut für Biochemie, Technische Hochschule Darmstadt, FRG.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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