Linked Life Data

2789331

Source:http://linkedlifedata.com/resource/pubmed/id/2789331

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1989-10-23
pubmed:abstractText
Effect of reconstituted type I collagen gel on in vitro growth of fibroblasts was examined. A prolonged lag period was observed by culture either on the surface of three-dimensional collagen gel ("on-gel") or within the gels ("in-gel") as compared with the culture on plastic dishes. The rate of cell proliferation in logarithmic phase growth was repressed by the culture "in-gel" but not by the culture "on-gel". The differential growth rates between "in-gel" culture and "on-gel" culture should be ascribed to difference in distributions of interacting sites between cell and collagen fibrils. The repression of cell growth was more marked in a contracting collagen gel which contains higher density of collagen fibrils. The cell density in contracted gel per unit cross-section area was found to be much lower than that of confluent monolayer culture. These results suggest that the repression of cell growth by collagen fibrils in the three-dimensional gels is not due to direct cell-cell contact, but due to the distribution and number of contact sites between a cell and collagen fibrils.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0934-8832
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
193-9
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Growth rate of human fibroblasts is repressed by the culture within reconstituted collagen matrix but not by the culture on the matrix.
pubmed:affiliation
Shiseido Basic Research Laboratories, Yokohama, Japan.
pubmed:publicationType
Journal Article