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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
27
|
| pubmed:dateCreated |
1989-10-25
|
| pubmed:databankReference | |
| pubmed:abstractText |
Rat peritoneal exudate cells produce two interleukin 6 (IL6) messenger RNA species, a major 1200 nucleotide and a 5-fold less abundant, 2400-nucleotide species. A cDNA clone representing the major species was isolated, and sequenced. The 1055-base pair insert covered the 3'-nontranslated region, the 211 triplet coding region and most of the 5'-nontranslated region. The derived rat IL6 amino acid sequence was 93 and 58% identical, respectively, with mature murine and human IL6. Rat IL6 lacks N-glycosylation sites but contains a fifth cysteinyl residue in addition to the 4 residues shared in conserved positions with murine and human IL6. Stable murine L cell and human HeLa-derived cell lines were established by cotransfection with rat IL6 cDNA and a selectable neomycin resistance marker. These lines secrete 9-fold increased amounts of functional IL6 over their respective parental cells. A stable rat macrophage-derived cell line, RM-SV1, was established by transformation with simian virus 40. IL6 and Il1 mRNA levels are inducible 20- and 3.5-fold, respectively, in this line by treatment with lipopolysaccharide with kinetics characteristic of macrophages. A set of three overlapping genomic DNA clones was isolated and a 10-kilobase DNA segment was sequenced containing the rat IL6 gene plus 2.9 kilobases of 5'-flanking and 1.3 kilobases of 3'-flanking sequences. The two transcription start sites used in RM-SV1 cells were mapped within 5 base pairs of each other. The exon/intron boundaries are conserved with the murine and human IL6 genes. The two IL6 mRNA species are generated by alternative polyadenylation at sites separated by a distance of 1.2 kilobases. The intervening region contains a repetitive element 72-80% identical with the rat and murine consensus L1 family sequences.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
25
|
| pubmed:volume |
264
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
16072-82
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:2789217-Amino Acid Sequence,
pubmed-meshheading:2789217-Animals,
pubmed-meshheading:2789217-Base Sequence,
pubmed-meshheading:2789217-Cells, Cultured,
pubmed-meshheading:2789217-DNA,
pubmed-meshheading:2789217-Exons,
pubmed-meshheading:2789217-Genes,
pubmed-meshheading:2789217-Humans,
pubmed-meshheading:2789217-Interleukin-6,
pubmed-meshheading:2789217-Interleukins,
pubmed-meshheading:2789217-Introns,
pubmed-meshheading:2789217-Macrophages,
pubmed-meshheading:2789217-Male,
pubmed-meshheading:2789217-Molecular Sequence Data,
pubmed-meshheading:2789217-RNA, Messenger,
pubmed-meshheading:2789217-Rats,
pubmed-meshheading:2789217-Rats, Inbred F344,
pubmed-meshheading:2789217-Restriction Mapping,
pubmed-meshheading:2789217-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2789217-Software,
pubmed-meshheading:2789217-Transcription, Genetic
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Structure of the rat interleukin 6 gene and its expression in macrophage-derived cells.
|
| pubmed:affiliation |
Department of Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|