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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
17
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pubmed:dateCreated |
1989-10-6
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pubmed:databankReference |
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pubmed:abstractText |
A transcriptional enhancer element has been identified 4.5 kilobases 3' of C alpha (constant region alpha chain) in the human T-cell receptor (TCR) alpha-chain locus. This enhancer is active on both a TCR V alpha (variable region alpha chain) promoter and the minimal simian virus 40 promotor in TCR alpha/beta Jurkat and EL4 cells but is inactive on a V alpha promoter in human TCR gamma/delta PEER and Molt-13 cells, clone 13 B cells, and HeLa fibroblasts. The enhancer has been localized to a 116-base-pair BstXI/Dra I restriction enzyme fragment, which lacks immunoglobulin octamer and kappa B enhancer motifs but does contain a consensus cAMP-response element (CRE). DNase I footprint analyses demonstrated that the minimal enhancer contains two binding sites for Jurkat nuclear proteins. One of these sites corresponds to the CRE, while the other does not correspond to a known transcriptional enhancer motif. These data support a model in which TCR alpha gene transcription is regulated by a unique set of cis-acting sequences and trans-acting factors, which are differentially active in cells of the TCR alpha/beta lineage. In addition, the TCR alpha enhancer may play a role in activating oncogene expression in T-lymphoblastoid tumors that have previously been shown to display chromosomal translocations into the human TCR alpha locus.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-2426193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-2524381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-2761540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-2822255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-2838755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-2875459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-2928113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-2968651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-2983227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-3029703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-3040262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-3043226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-3050531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-3091258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-3092224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-3186718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-3261760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-3261843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-3265470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-3266655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-3317824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-3396541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-3501368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-3517860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-3755221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-3871511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-3875152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-3912169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-3919308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-6425835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-6440030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-6828386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2788889-6960240
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6714-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2788889-Base Sequence,
pubmed-meshheading:2788889-Cloning, Molecular,
pubmed-meshheading:2788889-Enhancer Elements, Genetic,
pubmed-meshheading:2788889-Genes, Immunoglobulin,
pubmed-meshheading:2788889-Humans,
pubmed-meshheading:2788889-Immunoglobulin Constant Regions,
pubmed-meshheading:2788889-Immunoglobulin Variable Region,
pubmed-meshheading:2788889-Molecular Sequence Data,
pubmed-meshheading:2788889-Plasmids,
pubmed-meshheading:2788889-Receptors, Antigen, T-Cell,
pubmed-meshheading:2788889-Restriction Mapping,
pubmed-meshheading:2788889-T-Lymphocytes,
pubmed-meshheading:2788889-Transcription, Genetic,
pubmed-meshheading:2788889-Transfection
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pubmed:year |
1989
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pubmed:articleTitle |
A T-cell-specific transcriptional enhancer element 3' of C alpha in the human T-cell receptor alpha locus.
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pubmed:affiliation |
Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor 48109.
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pubmed:publicationType |
Journal Article
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