Linked Life Data

2787994

Source:http://linkedlifedata.com/resource/pubmed/id/2787994

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1989-9-21
pubmed:abstractText
The different methods proposed for preparation of B lymphocyte suspensions are not always simple, rapid and reliable. Presence of monocytes in the B cell suspension makes difficult HLA-DQ typing. For these reasons we propose a method using monoclonal antibodies (McAb) and complement to lyse the non B cells. Mononuclear cells, isolated from peripheral blood by Ficoll-Hypaque, are suspended in a mixture of anti-CD2, CD3, CD11b (OKM1 recognizing monocytes and granulocytes) McAb. This method of preparation of B cell suspension is rapid and provide better typing reactions than did the suspensions prepared by depletion of sheep red blood cell rosetting cells.
pubmed:language
fre
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1140-4639
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
135-40
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
[HLA class II typing of peripheral blood B lymphocytes separated using monoclonal antibodies].
pubmed:affiliation
Laboratoire d'Immunologie et d'Histocompatibilité, Hôpital Saint-Louis, Paris.
pubmed:publicationType
Journal Article, Comparative Study, English Abstract