Linked Life Data

2787693

Source:http://linkedlifedata.com/resource/pubmed/id/2787693

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1989-9-1
pubmed:abstractText
Mammalian cells infected with retroviruses frequently display virus particles budding at the tip of cellular projections resembling microvilli and filopodia. In normal and infected cells these cellular projections contain actin microfilaments and in specialized retrovirus-tipped projections from the P815 cell, a direct association between actin filaments and the apical virus particle could be demonstrated (Mortara and Koch, 1986). Here we confirm and extend these observations using a murine macrophage cell line chronically infected with a C-type retrovirus. Immunochemical and biochemical methods were used to identify actin-associated and actin-binding components among the retroviral polypeptides. The results show that Pr65gag and its p15 N-terminal domain can bind to actin in vitro and may be major binding sites for actin filaments on the retroviral nucleocapsid.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1122-9497
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
295-306
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
An association between actin and nucleocapsid polypeptides in isolated murine retroviral particles.
pubmed:affiliation
Medical Research Council, Laboratory of Molecular Biology, Cambridge, U.K.
pubmed:publicationType
Journal Article