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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
14
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pubmed:dateCreated |
1989-8-16
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pubmed:abstractText |
Interleukin 1 (IL-1) is a lymphokine secreted by monocytes in response to a variety of inflammatory stimuli. IL-1 beta, the predominant form of IL-1 produced by human monocytes, is synthesized as an inactive precursor of 31 kDa and is cleaved at Asp116-Ala117 to yield a 17.5-kDa extracellular form. The exact cellular site of cleavage and mechanism of secretion is at present unknown. We have prepared cell-free postnuclear extracts from freshly isolated human monocytes as well as THP.1 cells, a human monocyte-like cell line, and various blood lymphocytes and fibroblast cell lines. Using pre-IL-1 beta synthesized by in vitro transcription and translation, we have shown that only extracts derived from human monocytes and THP.1 cells were capable of cleaving precursor IL-1 beta to authentic mature IL-1 beta. Subcellular fractionation of the extracts suggested that the processing activity is found in the cytosol of monocytes or monocyte-like cell lines. The cleavage product of this protease is identical to authentic IL-1 beta as shown by mobility on SDS/PAGE and amino acid sequence analysis of the [3H]leucine-labeled product. The cleavage product is also capable of binding to the IL-1 receptor found on fibroblast membranes. Finally, mutation of Asp116----Ala116 rendered the IL-1 beta precursor resistant to cleavage by the processing activity. We conclude that a protease activity found only in monocytes will specifically process IL-1 beta to an active form.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-13786642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-212560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-2449207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-2578046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-2842866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-2950091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-2989698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-3047139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-3259579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-3261832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-3279153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-3288634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-3488079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-3491144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-3492551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-3494541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-3517219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-3520562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-3611052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-3636599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-3875682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-455458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-6083565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-6209582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-6309415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2787508-6970727
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5227-31
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2787508-Caspase 1,
pubmed-meshheading:2787508-Cell Line,
pubmed-meshheading:2787508-Cell-Free System,
pubmed-meshheading:2787508-Cells, Cultured,
pubmed-meshheading:2787508-Centrifugation, Density Gradient,
pubmed-meshheading:2787508-Humans,
pubmed-meshheading:2787508-Interleukin-1,
pubmed-meshheading:2787508-Metalloendopeptidases,
pubmed-meshheading:2787508-Monocytes,
pubmed-meshheading:2787508-Mutation,
pubmed-meshheading:2787508-Protein Precursors
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pubmed:year |
1989
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pubmed:articleTitle |
Identification of a monocyte specific pre-interleukin 1 beta convertase activity.
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pubmed:affiliation |
Department of Biochemical and Molecular Pathology, Merck Sharp & Dohme Research Laboratories, Rahway, NJ 07065.
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pubmed:publicationType |
Journal Article
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