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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1989-5-31
|
| pubmed:abstractText |
The pathogenesis of SLE is commonly attributed to the deposition of circulating immune complexes consisting of DNA and anti-DNA autoantibodies. However, recent work has shown multiple cross-reactions between anti-DNA antibodies and a variety of cellular and extracellular Ag. To test the possibility that these antibodies interact directly with glomerular Ag and induce kidney dysfunction, we applied mouse and human anti-DNA IgG to the isolated perfused rat kidney. The NZB/NZW mouse monoclonal anti-DNA bound to glomerular Ag with a concomitant induction of proteinuria and a decrease in inulin clearance. The albumin excretion was 2301 +/- 734 micrograms/min at 160 min of perfusion, as compared with 85 +/- 21 micrograms/min in controls (p less than 0.001). The inulin clearance was reduced to 0.17 +/- 0.02 ml/min as compared with 0.28 +/- 0.09 ml/min in controls (p less than 0.05). Polyclonal anti-DNA IgG obtained from patients with lupus nephritis bound to rat glomeruli and induced albumin excretion of 542 +/- 217 micrograms/min at 160 min of perfusion, as compared with 163 +/- 77 micrograms/min in controls (p = NS). The addition of plasma as a source of C to the human IgG increased the proteinuria markedly (albumin excretion of 1115 +/- 195 micrograms/min at 160 min of perfusion, p less than 0.02), probably due to C activation. Preincubation of the reactive mouse and human IgG with DNA completely abolished their binding to renal tissue and its physiologic consequences. These results suggest that direct binding of anti-DNA antibodies to renal Ag may play an important role in the induction of lupus nephritis.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Antinuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Autoantigens,
http://linkedlifedata.com/resource/pubmed/chemical/Complement System Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
142
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3076-82
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:2785132-Adolescent,
pubmed-meshheading:2785132-Adult,
pubmed-meshheading:2785132-Animals,
pubmed-meshheading:2785132-Antibodies, Antinuclear,
pubmed-meshheading:2785132-Autoantigens,
pubmed-meshheading:2785132-Binding Sites, Antibody,
pubmed-meshheading:2785132-Complement System Proteins,
pubmed-meshheading:2785132-DNA,
pubmed-meshheading:2785132-Female,
pubmed-meshheading:2785132-Humans,
pubmed-meshheading:2785132-Immunoglobulin G,
pubmed-meshheading:2785132-Kidney,
pubmed-meshheading:2785132-Lupus Erythematosus, Systemic,
pubmed-meshheading:2785132-Male,
pubmed-meshheading:2785132-Mice,
pubmed-meshheading:2785132-Mice, Inbred BALB C,
pubmed-meshheading:2785132-Middle Aged,
pubmed-meshheading:2785132-Perfusion,
pubmed-meshheading:2785132-Plasma,
pubmed-meshheading:2785132-Rats,
pubmed-meshheading:2785132-Rats, Inbred Strains
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Anti-DNA antibodies bind directly to renal antigens and induce kidney dysfunction in the isolated perfused rat kidney.
|
| pubmed:affiliation |
Department of Medicine, Hadassah University Hospitals, Jerusalem, Israel.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|