Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1989-4-17
|
| pubmed:abstractText |
B2A2-CD4-8- cells represent a rare subpopulation of thymocytes normally comprising 0.5% of the total adult thymus. These cells are known to express CD3-associated T cell receptor (TcR) alpha/beta molecules. In the present study we have examined the functional capacity of alpha/beta heterodimers on B2A2-CD4-8- cells. In the presence of monoclonal antibody (mAb) specific for either murine CD3 or TcR expressing the V beta 8-encoded beta chain (F23.1), B2A2-CD4-8- cells proliferated. Such proliferation was blocked by mAb to interleukin 2 receptor (IL 2R), suggesting an autocrine mechanism involving IL 2 production and subsequent utilization. IL 2 and also IL 3 production by mAb-stimulated B2A2-CD4-8- cells was directly confirmed. Furthermore, a panel of B2A2-CD4-8- clones were derived to assess the role of the TcR in cytolysis. Many clones were isolated which killed Fc receptor-bearing P815 target cells only in the presence of F23.1 mAb. Finally, in vivo treatment of neonatal mice with F23.1 mAb resulted in a marked reduction of V beta 8+ B2A2-CD4-8- thymocytes. Collectively, these results indicate that the TcR alpha/beta complex on CD4-CD8-B2A2- cells is fully capable of transducing signals that lead to proliferation, lymphokine production and cytolysis in vitro, as well as to disappearance of this subset from the thymus in vivo.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0014-2980
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
19
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
25-30
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:2784108-Animals,
pubmed-meshheading:2784108-Antibodies, Monoclonal,
pubmed-meshheading:2784108-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:2784108-Binding Sites, Antibody,
pubmed-meshheading:2784108-Cytotoxicity, Immunologic,
pubmed-meshheading:2784108-Female,
pubmed-meshheading:2784108-Interleukin-2,
pubmed-meshheading:2784108-Lymphocyte Activation,
pubmed-meshheading:2784108-Mice,
pubmed-meshheading:2784108-Mice, Inbred BALB C,
pubmed-meshheading:2784108-Mice, Inbred C57BL,
pubmed-meshheading:2784108-Phenotype,
pubmed-meshheading:2784108-Receptors, Antigen, T-Cell,
pubmed-meshheading:2784108-T-Lymphocytes, Cytotoxic
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Functional responsiveness in vitro and in vivo of alpha/beta T cell receptors expressed by the B2A2 (J11d)- subset of CD4-8- thymocytes.
|
| pubmed:affiliation |
Ludwig Institute for Cancer Research, Lausanne Branch, Epalinges, Switzerland.
|
| pubmed:publicationType |
Journal Article
|