Linked Life Data

2783206

Source:http://linkedlifedata.com/resource/pubmed/id/2783206

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
1989-10-26
pubmed:abstractText
We describe a convenient method for the separation and quantitation of plasma free cholesterol and cholesteryl esters by high performance liquid chromatography (HPLC). After extraction of 100 microliters plasma with isopropanol the plasma cholesteryl esters were resolved on a Zorbax ODS reversed-phase column by isocratic elution with acetonitrile/isopropanol (50:50, v/v). Baseline separation of the plasma cholesteryl esters including the internal standard was obtained within a 25-min run. The intra- and interassay CV was less than 4%. The results obtained by HPLC show good agreement with enzymatic and gas-liquid chromatographic methods. High performance liquid chromatography provides a simple method for the quantitation of individual cholesteryl esters avoiding tedious chromatographic and derivatisation steps inherent to GLC. Our HPLC method was applied to the monitoring of plasma cholesteryl esters in a normal population and can also be used for the study of cholesteryl esters from lipid extracts of biological samples.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9150
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
245-50
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Quantitation of plasma free cholesterol and cholesteryl esters by high performance liquid chromatography. Study of a normal population.
pubmed:affiliation
Department of Clinical Chemistry, A.Z. St-Jan, Bruges, Belgium.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't