| pubmed-article:2779577 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2779577 | lifeskim:mentions | umls-concept:C0446013 | lld:lifeskim |
| pubmed-article:2779577 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:2779577 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:2779577 | lifeskim:mentions | umls-concept:C0332453 | lld:lifeskim |
| pubmed-article:2779577 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:2779577 | pubmed:dateCreated | 1989-10-26 | lld:pubmed |
| pubmed-article:2779577 | pubmed:abstractText | We have demonstrated that genes from Ustilago maydis can be cloned by direct complementation of mutants through the use of genomic libraries made in a high-frequency transformation vector. We isolated a gene involved in amino acid biosynthesis as an illustrative example and showed that integrative and one-step disruption methods can be used to create null mutations in the chromosomal copy of the gene by homologous recombination. The results of this investigation make it clear that one-step gene disruption will be of general utility in investigations of U. maydis, since simple, precise replacement of the sequence under study was readily achieved. | lld:pubmed |
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| pubmed-article:2779577 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:2779577 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2779577 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2779577 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:2779577 | pubmed:issn | 0270-7306 | lld:pubmed |
| pubmed-article:2779577 | pubmed:author | pubmed-author:HollomanW KWK | lld:pubmed |
| pubmed-article:2779577 | pubmed:author | pubmed-author:FotheringhamS... | lld:pubmed |
| pubmed-article:2779577 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2779577 | pubmed:volume | 9 | lld:pubmed |
| pubmed-article:2779577 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2779577 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2779577 | pubmed:pagination | 4052-5 | lld:pubmed |
| pubmed-article:2779577 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:2779577 | pubmed:meshHeading | pubmed-meshheading:2779577-... | lld:pubmed |
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| pubmed-article:2779577 | pubmed:meshHeading | pubmed-meshheading:2779577-... | lld:pubmed |
| pubmed-article:2779577 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2779577 | pubmed:articleTitle | Cloning and disruption of Ustilago maydis genes. | lld:pubmed |
| pubmed-article:2779577 | pubmed:affiliation | Interdivisional Program in Molecular Biology, Graduate School of Medical Sciences, Cornell University Medical College, New York, New York 10021. | lld:pubmed |
| pubmed-article:2779577 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2779577 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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