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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1989-10-26
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pubmed:abstractText |
We have demonstrated that genes from Ustilago maydis can be cloned by direct complementation of mutants through the use of genomic libraries made in a high-frequency transformation vector. We isolated a gene involved in amino acid biosynthesis as an illustrative example and showed that integrative and one-step disruption methods can be used to create null mutations in the chromosomal copy of the gene by homologous recombination. The results of this investigation make it clear that one-step gene disruption will be of general utility in investigations of U. maydis, since simple, precise replacement of the sequence under study was readily achieved.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2779577-2829206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2779577-2851726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2779577-2907604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2779577-3319781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2779577-4059056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2779577-4932892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2779577-5878282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2779577-6214313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2779577-6310324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2779577-6312838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2779577-6319235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2779577-7046050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2779577-788919
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4052-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2779577-Basidiomycota,
pubmed-meshheading:2779577-Cloning, Molecular,
pubmed-meshheading:2779577-Genes, Fungal,
pubmed-meshheading:2779577-Genetic Complementation Test,
pubmed-meshheading:2779577-Genetic Markers,
pubmed-meshheading:2779577-Genetic Vectors,
pubmed-meshheading:2779577-Mutation,
pubmed-meshheading:2779577-Plasmids,
pubmed-meshheading:2779577-Transformation, Genetic,
pubmed-meshheading:2779577-Ustilago
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pubmed:year |
1989
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pubmed:articleTitle |
Cloning and disruption of Ustilago maydis genes.
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pubmed:affiliation |
Interdivisional Program in Molecular Biology, Graduate School of Medical Sciences, Cornell University Medical College, New York, New York 10021.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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