Linked Life Data

277912

Source:http://linkedlifedata.com/resource/pubmed/id/277912

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1978-10-25
pubmed:abstractText
The conjugative TOL plasmid (75 Mdal), specifying biodegradation of xylenes, toluene, and trimethylbenzene derivatives, undergoes dissociation in Pseudomonas aeruginosa PAO to a nonconjugative TOL(*) plasmid (28 Mdal) and a transfer plasmid termed TOLDelta (48 Mdal). The TOL(*) plasmid is rendered transmissible through introduction of a number of conjugative plasmids such as factor K, CAM, and TOLDelta but not by the FP2 derivative pR0271. Transfer of TOL(*) via factor K or TOLDelta is mediated by the formation of plasmid cointegrates; no recombination is observed with CAM. A recombinant RP4-TOL plasmid (76 Mdal), which has lost resistance to tetracycline, has been isolated. The TOL(*) segment can be transposed from this RP4-TOL recombinant plasmid to other antibiotic resistance plasmids such as R702. A segment of DNA, specifying salicylate degradation from SAL plasmid, was transposed onto pAC10, the TOL(*-) derivative of RP4-TOL recombinant plasmid, which has lost resistance to tetracycline but retains the transfer genes of RP4. Transposition of the salicylate degradative genes onto pAC10 results in the loss of kanamycin resistance. It has been possible to isolate SAL(+) segregants from pAC10[unk]SAL transposition derivatives that have lost the pAC10 plasmid. Such segregants harbor the salicylate degradative genes in the form of a nonconjugative plasmid (SAL(*)). Transfer of RP4[unk]TOL(*) or pAC10[unk]SAL(*) transposition derivatives to Escherichia coli, Salmonella typhimurium, Agrobacterium tumefaciens, or Azotobacter vinelandii results in the functional expression of the antibiotic resistance genes but not of the hydrocarbon degradative genes. Such genes, however, are fully expressed on being transferred back to Pseudomonas.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/277912-1092667, http://linkedlifedata.com/resource/pubmed/commentcorrection/277912-1092669, http://linkedlifedata.com/resource/pubmed/commentcorrection/277912-1103151, http://linkedlifedata.com/resource/pubmed/commentcorrection/277912-322140, http://linkedlifedata.com/resource/pubmed/commentcorrection/277912-326413, http://linkedlifedata.com/resource/pubmed/commentcorrection/277912-418059, http://linkedlifedata.com/resource/pubmed/commentcorrection/277912-792710, http://linkedlifedata.com/resource/pubmed/commentcorrection/277912-797315, http://linkedlifedata.com/resource/pubmed/commentcorrection/277912-797531, http://linkedlifedata.com/resource/pubmed/commentcorrection/277912-895716, http://linkedlifedata.com/resource/pubmed/commentcorrection/277912-895872, http://linkedlifedata.com/resource/pubmed/commentcorrection/277912-956125
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3109-12
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Transposition of plasmid DNA segments specifying hydrocarbon degradation and their expression in various microorganisms.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.