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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1989-10-17
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pubmed:abstractText |
Different factors that give the maximum iodination of proteins by iodogen method have been evaluated. The quantity of iodogen was varied from 2 to 20 micrograms; pH from 3 to 9; the quantity of bovine serum albumin (BSA) from 25 micrograms to 2 mg; the 131I activity from 200 microCi to 5 mCi and the reaction time from 2 to 30 min. The maximum iodination of proteins is achieved with iodogen-to-iodide molar ratio of 100-200 and iodogen-to-protein molar ratio of 1-2 at pH 6-8 for 10-15 min at room temperature.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,3,4,6-tetrachloro-3 alpha,6...,
http://linkedlifedata.com/resource/pubmed/chemical/Indicators and Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Serum Albumin, Bovine,
http://linkedlifedata.com/resource/pubmed/chemical/Serum Albumin, Radio-Iodinated,
http://linkedlifedata.com/resource/pubmed/chemical/Urea
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pubmed:status |
MEDLINE
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pubmed:issn |
0883-2897
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
431-3
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pubmed:dateRevised |
2008-2-21
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pubmed:meshHeading |
pubmed-meshheading:2777585-Animals,
pubmed-meshheading:2777585-Cattle,
pubmed-meshheading:2777585-Humans,
pubmed-meshheading:2777585-Hydrogen-Ion Concentration,
pubmed-meshheading:2777585-Indicators and Reagents,
pubmed-meshheading:2777585-Isotope Labeling,
pubmed-meshheading:2777585-Serum Albumin, Bovine,
pubmed-meshheading:2777585-Serum Albumin, Radio-Iodinated,
pubmed-meshheading:2777585-Urea
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pubmed:year |
1989
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pubmed:articleTitle |
Conditions of radioiodination with iodogen as oxidizing agent.
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pubmed:affiliation |
Department of Nuclear Medicine, Cleveland Clinic Foundation, OH 44195-5074.
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pubmed:publicationType |
Journal Article
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