Linked Life Data

2776132

Source:http://linkedlifedata.com/resource/pubmed/id/2776132

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1989-10-24
pubmed:abstractText
Several oligosaccharides from human milk were separated completely on a Dionex AS6 ion-exchange column under mild alkaline conditions, such that no degradation of oligosaccharides was detectable after incubation for 6 h at room temperature in the eluent buffer. In general, both the presence of fucosyl groups and branching within oligosaccharide chains tend to reduce retention times for oligosaccharides in this system. Thus, both lacto-N-fucopentaose II [beta-D-Galp-(1----3)-[alpha-L-Fucp-(1----4)]-beta-D-GlcpNAc-(1--- -3)- beta-D-Galp-(1----4)-D-Glc] and lacto-N-fucopentaose III [beta-D-Galp-(1----4)-[alpha-L-Fucp-(1----3)]-beta-D-GlcpNAc-(1--- -3)- beta-D-Galp-(1----4)-D-Glc] were eluted before lacto-N-fucopentaose I [alpha-L-Fucp-(1----2)-beta-D-Galp-(1----3)-beta-D-GlcpNAc-(1----3 )-beta-D- Galp-(1----4)-D-Glc], and all three fucopentaoses were eluted earlier than lacto-N-tetraose [beta-D-Galp-(1----3)-beta-D-GlcpNAc-(1----3)-beta-D-Galp-(1----4)-D-Glc ]. 2'-Fucosyllactose [alpha-L-Fucp-(1----2)-beta-D-Galp-(1----4)-D-Glc] and 3-fucosyllactose [alpha-D-Galp-(1----4)-[alpha-L-Fucp-(1----3)]-D-Glc] were separated completely under a wide range of conditions. Introduction of substituents at either the O-3 or O-4 of 2-acetamido-2-deoxy-D-glucose produced large differences in retention: lacto-N-tetraose and lacto-N-neotetraose [beta-D-Gal-(1----4)-beta-D-GlcpNAc-(1----3)-beta-D-Galp-(1----4)-D-Glc] could be separated by more than 8 min. The l.c. ion-exchange chromatographic system described represents a useful addition to existing methods for separating oligosaccharides derived from glycoproteins and glycolipids.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0008-6215
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
189
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-11
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Liquid ion-exchange chromatography under pressure of milk oligosaccharides using a pulsed amperometric detector.
pubmed:affiliation
Laboratory of Pathology, National Cancer Institute, Bethesda, Maryland 20892.
pubmed:publicationType
Journal Article