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rdf:type |
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lifeskim:mentions |
umls-concept:C0007610,
umls-concept:C0008633,
umls-concept:C0008643,
umls-concept:C0008672,
umls-concept:C0017337,
umls-concept:C0033684,
umls-concept:C0123921,
umls-concept:C0475264,
umls-concept:C1283195,
umls-concept:C1510827,
umls-concept:C1516050
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pubmed:issue |
15
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pubmed:dateCreated |
1989-9-29
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pubmed:abstractText |
Three human mRNAs are regulated post-transcriptionally by iron via iron-responsive elements (IREs) contained in each mRNA. A cytoplasmic protein (IRE-BP) binds to these cis-acting elements and mediates the translational regulation of ferritin H- and L-chain mRNA and the iron-dependent stability of transferrin receptor (TfR) mRNA. We have taken advantage of the different mobilities of the human and rodent IRE/IRE-BP complexes on non-denaturing polyacrylamide gels to determine the chromosomal localization of the gene encoding the IRE-BP. Utilizing a panel of 34 different human/rodent hybrid cell lines we have assigned the IRE-BP gene to human chromosome 9. This new technique based on nucleic acid/protein interaction may allow determination of the chromosomal localization of other RNA- or DNA-binding proteins.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2771641-204246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2771641-2452485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2771641-3020541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2771641-3127826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2771641-3266604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2771641-3413484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2771641-3477805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2771641-3479802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2771641-3685996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2771641-3857215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2771641-507053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2771641-6843651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2771641-7063884
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6103-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2771641-Animals,
pubmed-meshheading:2771641-Base Sequence,
pubmed-meshheading:2771641-Carrier Proteins,
pubmed-meshheading:2771641-Chromosome Mapping,
pubmed-meshheading:2771641-Chromosomes, Human, Pair 9,
pubmed-meshheading:2771641-Cricetinae,
pubmed-meshheading:2771641-Electrophoresis,
pubmed-meshheading:2771641-Ferritins,
pubmed-meshheading:2771641-Humans,
pubmed-meshheading:2771641-Hybrid Cells,
pubmed-meshheading:2771641-Iron,
pubmed-meshheading:2771641-Mice,
pubmed-meshheading:2771641-Plasmids,
pubmed-meshheading:2771641-Protein Biosynthesis,
pubmed-meshheading:2771641-RNA, Messenger,
pubmed-meshheading:2771641-RNA Probes,
pubmed-meshheading:2771641-Receptors, Transferrin,
pubmed-meshheading:2771641-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:2771641-Transcription, Genetic,
pubmed-meshheading:2771641-Tumor Cells, Cultured
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pubmed:year |
1989
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pubmed:articleTitle |
Chromosomal localization of nucleic acid-binding proteins by affinity mapping: assignment of the IRE-binding protein gene to human chromosome 9.
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pubmed:affiliation |
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892.
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pubmed:publicationType |
Journal Article
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