Linked Life Data

2767333

Source:http://linkedlifedata.com/resource/pubmed/id/2767333

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1989-9-28
pubmed:abstractText
Using the calcium-specific, chemiluminescent photoprotein aequorin, we have measured changes in the concentration of free cytosolic calcium at fertilization in single eggs of the ascidians Phallusia mammillata and Ciona intestinalis. Shortly after insemination, the free calcium concentration rises within a minute from a resting level of about 90 nM in the unfertilized egg to a peak level of about 7 microM in Phallusia and about 10 microM in Ciona. The total duration time of this fertilization transient is 2-3 min. It is immediately followed by a series of 12 to 25 briefer calcium transients with peak levels of about 1-4 microM. These postfertilization pulses occur at regular intervals of 1-3 min during the completion of meiosis, and they stop as soon as the second polar body is formed at about 25 min. An interesting exception to this pattern was observed in eggs from Ciona that had been raised at lower temperatures during the winter months. Insemination in the absence of external calcium in Phallusia results in a pulse pattern very similar to the normal pattern. From this result we infer that the bulk (if not all) of the calcium required for both the fertilization pulse and the meiotic oscillations is released from internal sources.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0012-1606
pubmed:author
pubmed:issnType
Print
pubmed:volume
135
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
182-90
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Free calcium pulses following fertilization in the ascidian egg.
pubmed:affiliation
Marine Biological Laboratory, Woods Hole, Massachusetts 02543.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't