Linked Life Data

2765628

Source:http://linkedlifedata.com/resource/pubmed/id/2765628

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1989-10-12
pubmed:abstractText
The correlation between the effects of pH, ionic strength and cation valency on the electrophoretic mobility and the extent of adhesion of suspension-cultured Catharanthus roseus cells to various polymer substrates is presented. The electrophoretic mobility of cells was unaltered in the pH range of 6-8, but decreased from approximately -2.2 x 10(-8) m V-1 s-1 and approached zero as the pH of the suspending liquid was decreased from 6 to 2. Similarly, the value of electrophoretic mobility decreased continuously as the ionic strength was increased from 0 to 1.0 M when cells were suspended in salt solutions of sodium chloride, calcium chloride, and aluminum chloride. However, using equimolar concentrations, the slope of the decrease in electrophoretic mobility increased following the sequence sodium chloride less than calcium chloride less than aluminium chloride. The electrophoretic mobility was near zero for suspensions containing 1.0 M calcium chloride or 0.1 M aluminium chloride. The extent of adhesion of the cells to the polymers sulphonated polystyrene less than polyethylene terephthalate less than polystyrene less than fluorinated ethylene-propylene followed this sequence. These results agree with a thermodynamic model of plant cell adhesion that implicates the importance of interfacial tensions in the adhesion process. However, higher levels of adhesion were generally observed when the electrophoretic mobility for the cells in the corresponding test liquid was at a minimum absolute value. These results can be explained by considering the effects of the electrolytic properties of the suspending liquid on the electrostatic repulsive interactions between the cells and the polymer surface in terms of a double-layer phenomenon and the DLVO theory.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0142-9612
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
318-24
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Adhesion of suspension-cultured Catharanthus roseus cells to surfaces: effect of pH, ionic strength, and cation valency.
pubmed:affiliation
Department of Botany, University of Toronto, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't