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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
15
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pubmed:dateCreated |
1989-9-12
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pubmed:abstractText |
We were interested in screening a series of isolates of the protozoan Leishmania for the presence of viruses. The experimental procedure we used was based on an enzymatic assay originally developed for viral RNA-dependent RNA polymerases. Simultaneously, total promastigote nucleic acid preparations were analyzed for the presence of viral genome and/or transcripts. Two isolates, both classified as L. braziliensis guyanensis, were found to be positive for RNA polymerase activity and to carry a large (6 kilobases) RNA species. The polymerase reaction products hybridized to the 6-kilobase RNA, believed to be the viral genome. In conjunction with electron microscopical observations these results indicate the presence of an RNA virus in these Leishmania isolates.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762308-16591471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762308-2436805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762308-2437529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762308-3200841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762308-3489942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762308-3807947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762308-4167449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762308-4316300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762308-4316301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762308-6261960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762308-6481853
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5979-82
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
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pubmed:year |
1989
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pubmed:articleTitle |
Characterization of a RNA virus from the parasite Leishmania.
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pubmed:affiliation |
Division of Infectious Diseases, Children's Hospital, Boston, MA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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