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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
1989-9-11
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pubmed:abstractText |
Transcription factor hTFIIIC was purified from cytoplasmic extracts of HeLa cells using four different chromatographic steps. This procedure yields a protein fraction which actively supports transcription in reconstitution assays and contains five major polypeptide chains with a molecular mass ranging from 25 to 250 kDa as estimated by SDS-PAGE and silver staining. In this fraction a polypeptide with a molecular mass of approximately 110 kDa could be identified as a specific DNA-binding component of hTFIIIC. By electrophoretic mobility shift and footprinting analyses it could be demonstrated that purified hTFIIIC binds specifically to the 5S gene. The protected region encompasses the A-Box promoter element and flanking sequences extending toward the 5'-proximal end of the gene. By addition of hTFIIIC to preformed TFIIIA/5S DNA complexes, we observe an additive effect of both factors on the footprint boundaries.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-11708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-212715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-2642897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-2649882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-2651441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-2664466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-2840349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-2862029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-2912980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-3052292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-3145406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-3375059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-3402432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-3417660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-3473469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-3512543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-3561399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-3561405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-3599075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-3633269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-3667626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-3691480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-3697084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-3791417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-4040853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-4414007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-6229542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-6233142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-6261953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-6319715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-6356356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-6530509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-6862025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-6943546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-7130190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-7440579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2762117-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal, 5S,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Transfer,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors, TFIII,
http://linkedlifedata.com/resource/pubmed/chemical/transcription factor TFIIIC
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5003-16
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2762117-Animals,
pubmed-meshheading:2762117-Chromatography, Affinity,
pubmed-meshheading:2762117-Chromatography, Ion Exchange,
pubmed-meshheading:2762117-Cytosol,
pubmed-meshheading:2762117-DNA,
pubmed-meshheading:2762117-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2762117-Genes,
pubmed-meshheading:2762117-HeLa Cells,
pubmed-meshheading:2762117-Humans,
pubmed-meshheading:2762117-Molecular Weight,
pubmed-meshheading:2762117-Plasmids,
pubmed-meshheading:2762117-Protein Binding,
pubmed-meshheading:2762117-RNA, Ribosomal,
pubmed-meshheading:2762117-RNA, Ribosomal, 5S,
pubmed-meshheading:2762117-RNA, Transfer,
pubmed-meshheading:2762117-Transcription Factors,
pubmed-meshheading:2762117-Transcription Factors, TFIII,
pubmed-meshheading:2762117-Xenopus laevis
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pubmed:year |
1989
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pubmed:articleTitle |
Purification of human transcription factor IIIC and its binding to the gene for ribosomal 5S RNA.
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pubmed:affiliation |
Institut für Molekularbiologie und Tumorforschung, Marburg, Lahn, FRG.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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