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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1989-9-21
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pubmed:databankReference |
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pubmed:abstractText |
The complete nucleotide sequence of the gene for a cell-surface protein antigen (PAc) of Streptococcus mutans MT8148 (serotype c) was determined. The pac gene consisted of 4695 bp and coded for a 170773D protein. The pac gene product contained a putative 38 amino acid signal peptide, resulting in a 166817D mature protein. A potential promoter sequence and a putative Shine-Dalgarno sequence preceded the open reading frame. Two internal repeating amino acid sequences were present in the PAc. One repeating region located in the N-terminal region was rich in alanine, and the other located in the central region was rich in proline. Southern blot analysis under the less stringent condition (allowing up to 35% base mismatch) revealed that the probe covering the proline-rich region hybridized to DNA preparations from strains of Streptococcus cricetus, Streptococcus sobrinus and Streptococcus downei as well as Streptococcus mutans.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0950-382X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
673-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:2761390-Amino Acid Sequence,
pubmed-meshheading:2761390-Antigens, Bacterial,
pubmed-meshheading:2761390-Antigens, Surface,
pubmed-meshheading:2761390-Base Sequence,
pubmed-meshheading:2761390-Blotting, Southern,
pubmed-meshheading:2761390-Codon,
pubmed-meshheading:2761390-DNA, Bacterial,
pubmed-meshheading:2761390-Dental Caries,
pubmed-meshheading:2761390-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2761390-Genes, Bacterial,
pubmed-meshheading:2761390-Membrane Proteins,
pubmed-meshheading:2761390-Molecular Sequence Data,
pubmed-meshheading:2761390-Molecular Weight,
pubmed-meshheading:2761390-Plasmids,
pubmed-meshheading:2761390-Promoter Regions, Genetic,
pubmed-meshheading:2761390-Protein Sorting Signals,
pubmed-meshheading:2761390-Restriction Mapping,
pubmed-meshheading:2761390-Serotyping,
pubmed-meshheading:2761390-Streptococcus mutans
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pubmed:year |
1989
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pubmed:articleTitle |
Molecular characterization of a surface protein antigen gene from serotype c Streptococcus mutans, implicated in dental caries.
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pubmed:affiliation |
Department of Dental Research, National Institute of Health, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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