Linked Life Data

2756937

Source:http://linkedlifedata.com/resource/pubmed/id/2756937

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1989-8-28
pubmed:abstractText
Patients on chronic hemodialysis who are treated with reusable dialyzers sterilized with formaldehyde are known to have antibodies develop with N-like specificity. These sera have been shown to also react with formaldehyde-treated N-negative cells. This latter specificity has been referred to as antiformaldehyde (anti-Form). Because anti-Form develops presumably in response to formaldehyde-treated cells that gain access to the patient's circulation, the authors reasoned that recipient anti-Form once established might be capable of binding to cells exposed to formaldehyde during dialysis, resulting in a positive direct antiglobulin test (DAT) result and an eluate reacting only with formaldehyde-treated reagent cells. The authors studied 96 patients on chronic hemodialysis who were exposed to formaldehyde-sterilized equipment. Both anti-N-like and anti-Form specificities were found. Anti-Form was detected in the serum of 16% of patients when tested against 0.37% (w/v) formaldehyde-treated cells. Nineteen percent of patients demonstrated a positive DAT result. The presence of a positive DAT result was significantly associated with the presence of anti-Form in the patient's serum (P less than 0.05). Red blood cell eluates from two patients with a positive IgG DAT result and serum anti-Form demonstrated anti-Form in the eluate. Increasing the concentration of formaldehyde treatment of red blood cells resulted in increasing strength of reactions with anti-Form serum, suggesting that the sensitivity of serum and eluate testing could be modified by the method of cell preparation. As expected, the reactivity of anti-Form with formaldehyde-treated cells could not be neutralized by formaldehyde. The authors conclude that patients treated with formaldehyde-sterilized reusable dialysis equipment may on occasion have a positive DAT result and have an eluate that is nonreactive with the use of standard reagent cells but reactive with formaldehyde-treated cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0002-9173
pubmed:author
pubmed:issnType
Print
pubmed:volume
92
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
214-7
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Positive direct antiglobulin test result in dialysis patients resulting from antiformaldehyde antibodies.
pubmed:affiliation
Department of Pathology, New England Deaconess Hospital, Boston, Massachusetts 02215.
pubmed:publicationType
Journal Article