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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1989-9-1
|
| pubmed:abstractText |
The initial phase of in vitro experiments in Ussing-type chambers on large intestine is characterized by short-circuit currents (ISC) declining from high starting values to a lower plateau within 0.5 h. The origin of this "initial ISC-transient" was investigated by ISC measurements on partially stripped segments of rat rectal colon. Transport was pre-stimulated in vivo by keeping animals in barbiturate-anesthesia for 5 h prior to tissue preparation. This procedure caused by endogenous aldosterone-liberation amiloride-sensitive Na-absorption to become the predominant electrogenic transport. The initial ISC-transient was abolished by tetrodotoxin (TTX, 1 microM), indicating a neuronal mediation of this phenomenon. In order to identify the transport which was subject to neuronal control, the amiloride-sensitive Na-absorption was measured during electrical field stimulation (bipolar rectangular pulses: 5 Hz, 1 ms, +/- 6 mA). There was no difference to unstimulated controls. In contrast, the initial ISC-transient was dependent on Cl in the bath following Michaelis-Menten-kinetics (KM = 20 mM) and could be prevented by 10 microM serosal bumetanide. Then, initial filling of the Ussing-chamber was imitated during the course of the experiment by removal and immediate re-addition of the bathing fluid. This procedure caused ISC-changes of similar appearance as the initial ISC-transient. To verify that indeed mechanical stretch is the sensory stimulus triggering the initial ISC-transient, the effect of small pressure oscillations was studied. This also produced an ISC-transient which was TTX-sensitive and was abolished after removal of the submucosal plexus Meissner by total stripping.(ABSTRACT TRUNCATED AT 250 WORDS)
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amiloride,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/Ions,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrodotoxin
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0031-6768
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
414
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
216-21
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:2755775-Absorption,
pubmed-meshheading:2755775-Amiloride,
pubmed-meshheading:2755775-Animals,
pubmed-meshheading:2755775-Biological Transport,
pubmed-meshheading:2755775-Chlorides,
pubmed-meshheading:2755775-Colon,
pubmed-meshheading:2755775-Electric Stimulation,
pubmed-meshheading:2755775-Electrophysiology,
pubmed-meshheading:2755775-Ions,
pubmed-meshheading:2755775-Male,
pubmed-meshheading:2755775-Nervous System,
pubmed-meshheading:2755775-Nervous System Physiological Phenomena,
pubmed-meshheading:2755775-Neurons,
pubmed-meshheading:2755775-Physical Stimulation,
pubmed-meshheading:2755775-Rats,
pubmed-meshheading:2755775-Rats, Inbred Strains,
pubmed-meshheading:2755775-Rectum,
pubmed-meshheading:2755775-Sodium,
pubmed-meshheading:2755775-Submucous Plexus,
pubmed-meshheading:2755775-Tetrodotoxin
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Ion transport and enteric nervous system (ENS) in rat rectal colon: mechanical stretch causes electrogenic Cl-secretion via plexus Meissner and amiloride-sensitive electrogenic Na-absorption is not affected by intramural neurons.
|
| pubmed:affiliation |
Medizinische Klinik, Abteilung für Gastroenterologie, Freie Universität Berlin.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|