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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1989-9-1
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pubmed:abstractText |
A novel bovine brain inositol-phospholipid-specific phospholipase C has been identified on the basis of chromatographic behaviour and purified to apparent homogeneity by a rapid three-step procedure. The purified enzyme has a molecular mass of 85 kDa on SDS/polyacrylamide gel electrophoresis and a specific activity of 24 mumol.min-1.mg-1. The enzyme is dependent on Ca2+ and shows a marked preference for inositol phospholipid substrates. The unique nature of this polypeptide was confirmed through partial protein sequence analysis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0014-2956
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
182
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
673-7
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:2753038-Amino Acid Sequence,
pubmed-meshheading:2753038-Animals,
pubmed-meshheading:2753038-Brain,
pubmed-meshheading:2753038-Cattle,
pubmed-meshheading:2753038-Chromatography,
pubmed-meshheading:2753038-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2753038-Hydrolysis,
pubmed-meshheading:2753038-Inositol Phosphates,
pubmed-meshheading:2753038-Molecular Sequence Data,
pubmed-meshheading:2753038-Peptide Fragments,
pubmed-meshheading:2753038-Peptides,
pubmed-meshheading:2753038-Phospholipids,
pubmed-meshheading:2753038-Sugar Phosphates,
pubmed-meshheading:2753038-Type C Phospholipases
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pubmed:year |
1989
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pubmed:articleTitle |
A novel inositol-phospholipid-specific phospholipase C. Rapid purification and characterization.
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pubmed:affiliation |
Ludwig Institute for Cancer Research, London, UK.
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pubmed:publicationType |
Journal Article,
Comparative Study
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