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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1989-8-17
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pubmed:abstractText |
The usefulness of lectin affinity chromatography for the preparation of glycoproteins is impaired by ligand release. Ligand leakage from mistletoe lectin (MLI) Sepharose 4B column was detected by 24 h skin reaction in mice and by immunoblotting. Immunoaffinity chromatography was found to be an efficient method for the separation of lectin traces from the glycoprotein fraction. A sugar concentration dependent increase of lectin release from MLI-Sepharose 4B column was detected by a solid phase enzyme immunoassay.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0232-766X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
48
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
221-6
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:2751632-Animals,
pubmed-meshheading:2751632-Chromatography, Affinity,
pubmed-meshheading:2751632-Fibrosarcoma,
pubmed-meshheading:2751632-Glycoproteins,
pubmed-meshheading:2751632-Lectins,
pubmed-meshheading:2751632-Ligands,
pubmed-meshheading:2751632-Mice,
pubmed-meshheading:2751632-Mice, Inbred CBA,
pubmed-meshheading:2751632-Mistletoe,
pubmed-meshheading:2751632-Plant Lectins,
pubmed-meshheading:2751632-Plant Preparations,
pubmed-meshheading:2751632-Plant Proteins,
pubmed-meshheading:2751632-Plants, Medicinal,
pubmed-meshheading:2751632-Ribosome Inactivating Proteins, Type 2,
pubmed-meshheading:2751632-Sarcoma, Experimental,
pubmed-meshheading:2751632-Skin Tests,
pubmed-meshheading:2751632-Toxins, Biological
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pubmed:year |
1989
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pubmed:articleTitle |
Detection and quantification of ligand leakage from lectin affinity columns.
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pubmed:affiliation |
Institute of Biochemistry, Wilhelm-Pieck University, Rostock, GDR.
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pubmed:publicationType |
Journal Article
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