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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1989-8-25
|
| pubmed:abstractText |
Enzymatic DNA amplification was applied to DNA and elementary bodies of C. trachomatis. Oligonucleotide primers were chosen in a sequence of a conserved domain of the major outer membrane protein to generate the amplification of a 129-base pair fragment. This sequence was amplified in the 15 serovars of C. trachomatis; however, serovar J gave a weaker signal than the others. The specificity was controlled by EcoRI restriction enzyme digestion and Southern analysis using an internal probe of the amplified sequence. No cross-reaction was shown with DNA of 11 other bacteria. Thus, enzymatic DNA amplification by the polymerase chain reaction appears to be a potential tool for the specific detection of C. trachomatis.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0923-2508
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
140
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
7-16
|
| pubmed:dateRevised |
2000-12-18
|
| pubmed:meshHeading |
pubmed-meshheading:2748993-Base Sequence,
pubmed-meshheading:2748993-Chlamydia trachomatis,
pubmed-meshheading:2748993-DNA, Bacterial,
pubmed-meshheading:2748993-Deoxyribonuclease EcoRI,
pubmed-meshheading:2748993-Gene Amplification,
pubmed-meshheading:2748993-Oligonucleotide Probes,
pubmed-meshheading:2748993-Species Specificity
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Specific amplification of a DNA sequence common to all Chlamydia trachomatis serovars using the polymerase chain reaction.
|
| pubmed:affiliation |
Laboratoire de Bactériologie, Hôpital Pellegrin, Bordeaux, France.
|
| pubmed:publicationType |
Journal Article
|