Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
1989-8-16
pubmed:abstractText
Recently, DNA sequences containing four erythroid-specific DNase I hypersensitive sites within 20 kilobases 5' of the human epsilon-globin gene have been identified as an important cis-acting regulatory element, the locus activation region (LAR). Subfragments of the LAR, containing either all or only the two 5' or two 3' hypersensitive sites were linked to the human beta-globin gene and analyzed for their effect on globin gene expression in stably transformed mouse erythroleukemia (MEL) cells. Constructs containing all four of the hypersensitive sites increase beta-globin mRNA levels 8- to 13-fold, while constructs with only the 5' or 3' sites increase globin expression to a lesser extent. No effect was seen when the constructs were assayed in 3T3 fibroblasts. All of the LAR derivatives form hypersensitive sites at the corresponding sequence position in MEL cells prior to and after induction of MEL cell differentiation. However, in 3T3 fibroblasts only the hypersensitive site corresponding to the previously described erythroid-specific -10.9 site was formed.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-2411209, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-2461328, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-2704733, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-2721958, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-2911581, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-2922063, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-2924354, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-293672, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-2992937, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-2997283, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-3029703, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-3039464, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-3288863, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-3413070, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-3456593, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-3465379, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-3478680, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-3480506, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-3690667, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-3858676, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-3879975, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-6088069, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-6155217, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-6200877, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-6205764, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-6286831, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-6318113, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-6572107, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-6578416, http://linkedlifedata.com/resource/pubmed/commentcorrection/2748594-7094019
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5439-43
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Molecular analysis of the human beta-globin locus activation region.
pubmed:affiliation
Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.