Linked Life Data

2743562

Source:http://linkedlifedata.com/resource/pubmed/id/2743562

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1989-8-15
pubmed:abstractText
Primary cultures from fetal rat hypothalamus (embryonic day 15-16) were grown for 9 days in a serum-free medium and then fixed and immunostained for glial fibrillary acidic protein (GFAP). The majority of the GFAP-immunoreactive astrocytes were flat, polygonal, without processes and showed a low intensity immunoreactivity which was restricted to the perinuclear region. Elongated, process-bearing astrocytes, with an intense immunoreactivity in the soma and processes, were also observed in a smaller proportion (30%). Addition of estradiol (10(-12) to 10(-8) M) to the culture medium 24 h before GFAP immunostaining resulted in an increased proportion (59-69%) of process-bearing, intense immunoreactive cells. This effect was blocked by tamoxifen (10(-8) M). The total number of GFAP-immunoreactive astrocytes was not modified by estrogen or tamoxifen. These results indicate that estradiol may modulate the cell shape and the distribution of GFAP in astrocytes in culture and suggest that astrocytes can be a target for sex steroids during development of the central nervous system.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0165-3806
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
298-302
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Astrocytic shape and glial fibrillary acidic protein immunoreactivity are modified by estradiol in primary rat hypothalamic cultures.
pubmed:affiliation
Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, CT 06510.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't