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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1989-7-24
|
| pubmed:abstractText |
A cDNA encoding the entire tau subunit of rabbit skeletal muscle phosphorylase kinase was reconstructed and inserted into a plasmid containing the Escherichia coli ptac promoter and a constructed plasmid containing the ptac promoter and bacterial chloramphenicol acetyl transferase (CAT) gene, respectively. A significant phosphorylase kinase activity was found, in the first case. In the second case, a fused protein containing 73 amino acids from the CAT protein was obtained. After renaturation, the CAT-tau subunit protein shows enzymatic activity similar to the HPLC-purified and renatured tau subunit.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0006-291X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
161
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
746-53
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2735920-Animals,
pubmed-meshheading:2735920-Blotting, Western,
pubmed-meshheading:2735920-Cloning, Molecular,
pubmed-meshheading:2735920-DNA,
pubmed-meshheading:2735920-Kinetics,
pubmed-meshheading:2735920-Macromolecular Substances,
pubmed-meshheading:2735920-Phosphorylase Kinase,
pubmed-meshheading:2735920-Plasmids,
pubmed-meshheading:2735920-Rabbits,
pubmed-meshheading:2735920-Recombinant Fusion Proteins,
pubmed-meshheading:2735920-Structure-Activity Relationship
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Expression and characterization of the tau subunit of phosphorylase kinase.
|
| pubmed:affiliation |
Dept. of Biochem. and Biophys., Iowa State Univ., Ames 50011.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|