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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1989-7-21
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pubmed:abstractText |
Vanadate stimulated the release of lipoprotein lipase (LPL) activity from rat fat pads into the medium in a time- and dose-dependent manner. It exerted the synergetic effect with heparin. The stimulatory effects of vanadate and heparin were decreased by incubation in Na+- or Ca2+-free media but were well preserved in K+-free medium. Amiloride inhibited the vanadate-stimulated release of LPL activity in a dose-dependent manner, but did not inhibit the heparin-stimulated release of LPL activity. Colchicine, antimycin A, and carbonyl cyanide m-chlorophenylhydrazone suppressed the stimulatory effect of vanadate, but cycloheximide did not. Preincubation of the fat pads with the tetrakis (acetoxymethyl) ester of quin 2 (quin 2-AM) inhibited the vanadate-stimulatory release of LPL activity without affecting basal activity. The concentration required for half-maximal inhibition of the action of vanadate by quin 2-AM was calculated to be 39 microM, suggesting that the action of vandate was dependent on intracellular Ca2+ concentration. The heparin-stimulated release, on the other hand, was not inhibited even at higher concentrations of quin 2-AM (up to 200 microM). These findings suggest that vanadate stimulates the release of LPL activity through mechanisms of action involving amiloride-sensitive and calcium-dependent pathways with a requirement of metabolic energy.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amiloride,
http://linkedlifedata.com/resource/pubmed/chemical/Aminoquinolines,
http://linkedlifedata.com/resource/pubmed/chemical/Antimycin A,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Carbonyl Cyanide m-Chlorophenyl...,
http://linkedlifedata.com/resource/pubmed/chemical/Chelating Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Colchicine,
http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide,
http://linkedlifedata.com/resource/pubmed/chemical/Heparin,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoprotein Lipase,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Quin2-acetoxymethyl ester,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium,
http://linkedlifedata.com/resource/pubmed/chemical/Vanadates
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0003-9861
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
272
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
18-24
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:2735761-Adipose Tissue,
pubmed-meshheading:2735761-Amiloride,
pubmed-meshheading:2735761-Aminoquinolines,
pubmed-meshheading:2735761-Animals,
pubmed-meshheading:2735761-Antimycin A,
pubmed-meshheading:2735761-Calcium,
pubmed-meshheading:2735761-Carbonyl Cyanide m-Chlorophenyl Hydrazone,
pubmed-meshheading:2735761-Chelating Agents,
pubmed-meshheading:2735761-Colchicine,
pubmed-meshheading:2735761-Cycloheximide,
pubmed-meshheading:2735761-Dose-Response Relationship, Drug,
pubmed-meshheading:2735761-Heparin,
pubmed-meshheading:2735761-Kinetics,
pubmed-meshheading:2735761-Lipoprotein Lipase,
pubmed-meshheading:2735761-Male,
pubmed-meshheading:2735761-Potassium,
pubmed-meshheading:2735761-Rats,
pubmed-meshheading:2735761-Rats, Inbred Strains,
pubmed-meshheading:2735761-Sodium,
pubmed-meshheading:2735761-Vanadates
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pubmed:year |
1989
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pubmed:articleTitle |
Stimulatory release of lipoprotein lipase activity from rat fat pads by vanadate.
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pubmed:affiliation |
Department of Biochemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Hiroshima, Japan.
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pubmed:publicationType |
Journal Article
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