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pubmed-article:2732088pubmed:abstractTextA non-radioactive in situ hybridization method using biotin-labelled rDNA has made it possible to localize rRNA genes not only at the secondary constriction in both homologous chromosomes No. 3 of Crepis capillaris but also in the B chromosomes occurring in the plants employed. Very clear dot-like rDNA signals at the telomeres of both arms were observed in all B chromosomes. Histochemical silver staining, which is indicative of transcriptional activity of rRNA gene clusters, resulted in both darkly-staining nucleolar constrictions of chromosomes No. 3 and silver deposits at the telomeres of Bs. We conclude that the B chromosomes of C. capillaris are isochromosomes with active rRNA genes located near both telomeres.lld:pubmed
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pubmed-article:2732088pubmed:authorpubmed-author:SchweizerDDlld:pubmed
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pubmed-article:2732088pubmed:year1989lld:pubmed
pubmed-article:2732088pubmed:articleTitleRibosomal RNA genes in B chromosomes of Crepis capillaris detected by non-radioactive in situ hybridization.lld:pubmed
pubmed-article:2732088pubmed:affiliationDepartment of Cytology & Genetics, University of Vienna, Austria.lld:pubmed
pubmed-article:2732088pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:2732088pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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