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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
1989-7-24
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pubmed:abstractText |
A non-radioactive in situ hybridization method using biotin-labelled rDNA has made it possible to localize rRNA genes not only at the secondary constriction in both homologous chromosomes No. 3 of Crepis capillaris but also in the B chromosomes occurring in the plants employed. Very clear dot-like rDNA signals at the telomeres of both arms were observed in all B chromosomes. Histochemical silver staining, which is indicative of transcriptional activity of rRNA gene clusters, resulted in both darkly-staining nucleolar constrictions of chromosomes No. 3 and silver deposits at the telomeres of Bs. We conclude that the B chromosomes of C. capillaris are isochromosomes with active rRNA genes located near both telomeres.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0018-067X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
62 ( Pt 1)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
59-65
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2732088-Chromosome Banding,
pubmed-meshheading:2732088-Chromosomes,
pubmed-meshheading:2732088-DNA Probes,
pubmed-meshheading:2732088-Fluorescence,
pubmed-meshheading:2732088-Interphase,
pubmed-meshheading:2732088-Metaphase,
pubmed-meshheading:2732088-Nucleic Acid Hybridization,
pubmed-meshheading:2732088-Operon,
pubmed-meshheading:2732088-Plants,
pubmed-meshheading:2732088-RNA, Ribosomal,
pubmed-meshheading:2732088-Seeds,
pubmed-meshheading:2732088-rRNA Operon
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pubmed:year |
1989
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pubmed:articleTitle |
Ribosomal RNA genes in B chromosomes of Crepis capillaris detected by non-radioactive in situ hybridization.
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pubmed:affiliation |
Department of Cytology & Genetics, University of Vienna, Austria.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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