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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1989-7-17
|
| pubmed:abstractText |
As part of our continuing studies into the biochemical basis of long-term changes in neuronal function in Aplysia, we have developed a simple method for obtaining amino acid sequence information from proteins isolated on two-dimensional gels. Proteins isolated on preparative two-dimensional gels are digested in situ with Staphylococcus aureus V8 protease, and the resulting peptides electrophoresed, transferred to a polyvinylidene difluoride membrane, and sequenced in a gas-phase sequencer. The method is simple and should be applicable to a variety of other systems where the development of a two-dimensional gel database is underway.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0173-0835
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
10
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
152-7
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2731514-Amino Acid Sequence,
pubmed-meshheading:2731514-Animals,
pubmed-meshheading:2731514-Aplysia,
pubmed-meshheading:2731514-Central Nervous System,
pubmed-meshheading:2731514-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:2731514-Information Systems,
pubmed-meshheading:2731514-Proteins,
pubmed-meshheading:2731514-Serine Endopeptidases
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Development of a database of amino acid sequences for proteins identified and isolated on two-dimensional polyacrylamide gels.
|
| pubmed:affiliation |
Howard Hughes Medical Institute, Center for Neurobiology and Behavior, College of Physicians and Surgeons of Columbia University, New York, NY 10032.
|
| pubmed:publicationType |
Journal Article
|