Linked Life Data

2730918

Source:http://linkedlifedata.com/resource/pubmed/id/2730918

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1989-7-27
pubmed:abstractText
Guinea pig biotinidase and lipoamidase were mostly located in the liver microsomal fraction. Approx. 80% of the total activities of both enzymes were associated with the membranes subfractions of liver. The subunit molecular masses of these enzymes as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of microsomal biotinidase and lipoamidase were 70 and 60 kDa, respectively. Sephadex G-200 gel-permeation chromatography in the presence of 0.1% Nonidet P-40 indicated that the native-state molecular masses of microsomal biotinidase and lipoamidase were 68 and 120 kDa, respectively. The isoelectric points of microsomal biotinidase and lipoamidase were 6.3 and 5.7, respectively. Linear sucrose density centrifugation analysis indicated that both enzymes exist in the rough endoplasmic reticulum. Comparison of amino acid analyses indicated a higher content of leucine and lower content of serine in lipoamidase than in biotinidase. Microsomal biotinidase and lipoamidase were classified as being thiol-type and serine-type amidases, respectively.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
991
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
410-4
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Biotinidase and lipoamidase in guinea pig livers.
pubmed:affiliation
Division of Metabolism, National Children's Medical Research Center, Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't