Linked Life Data

2725005

Source:http://linkedlifedata.com/resource/pubmed/id/2725005

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1989-7-7
pubmed:abstractText
A method for the quantitative assessment of peptide-containing nerves in the gastrointestinal muscle layers is described. Tissue samples are sectioned obliquely, at 45 degrees, in the plane bisecting the long axes of longitudinal and circular muscle fibres, and peptide-containing nerve fascicles are revealed by immunofluorescence. Cross-sectioned fascicles are counted (number/area of muscle) and their length density per volume of muscle layer is calculated. The composition of immunostained nerve fascicles is also assessed, by counting peptide-containing fibres in each, while peptide transmitter co-distributions are studied in parallel using adjacent tissue sections. Thus, the approach described provides a means for the delineation of both quantitative tissue distribution and composition of autonomic neuro-muscular junctions in the gastro-intestinal tract.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0165-0270
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
211-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Morphometry of peptide-containing nerves in gut muscle layers: a quantitative approach to the study of autonomic neuro-muscular junctions.
pubmed:affiliation
Department of Anatomy, Tor Vergata University, Rome, Italy.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't