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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1989-7-12
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| pubmed:abstractText |
We describe the development of radioimmunoassays to measure both human pepsinogen A and pepsinogen C concentrations in serum. The antibodies were raised in goats by immunization with purified pepsinogen A or C. The affinity constants of the respective antibodies were 20.10(10) l/mol and 7.10(10) l/mol. Pepsinogens A and C were labeled with Na 125I by the chloramine T method. The binding between labels and antibodies was inhibited by 0.50 at 0.82 ng pepsinogen A per tube and 2.1 ng pepsinogen C per tube. The detection limits of the assay of pepsinogen A and C were 0.12 microgram/l and 1.8 micrograms/l, respectively. Pepsinogen A and C were purified and added to a patient serum, showing a good recovery in the radioimmunoassays. Serial dilution of another patient serum, which contained a high concentration of both antigens, showed curves parallel to the standard curves. The intra- and interassay variations of these radioimmunoassays were evaluated. The intra-assay coefficients of variation for pepsinogen A were found to vary from 0.03 to 0.102 at concentrations in serum in the normal range, while the inter-assay coefficient of variation ranged from 0.118 to 0.194 at the same concentrations in serum. For the pepsinogen C radioimmunoassay we found intra-assay coefficients of variation between 0.126 and 0.147 at concentrations in serum in the normal range, while the inter-assay coefficient of variation ranged from 0.174 to 0.325 for the same sera. In 201 blood donors we found a mean serum concentration of pepsinogen A of 59 micrograms/l and a mean serum concentration of pepsinogen C of 15 micrograms/l. There was a significant relationship between these values (r = 0.779, p less than 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
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| pubmed:issn |
0340-076X
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
27
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
19-25
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| pubmed:dateRevised |
2007-7-24
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| pubmed:meshHeading |
pubmed-meshheading:2723588-Achlorhydria,
pubmed-meshheading:2723588-Adult,
pubmed-meshheading:2723588-Age Factors,
pubmed-meshheading:2723588-Duodenal Ulcer,
pubmed-meshheading:2723588-Female,
pubmed-meshheading:2723588-Gastrectomy,
pubmed-meshheading:2723588-Gastric Mucosa,
pubmed-meshheading:2723588-Humans,
pubmed-meshheading:2723588-Male,
pubmed-meshheading:2723588-Middle Aged,
pubmed-meshheading:2723588-Pepsinogens,
pubmed-meshheading:2723588-Radioimmunoassay
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Radioimmunoassay of human pepsinogen A and pepsinogen C.
|
| pubmed:affiliation |
Afdeling Maag-, Darm- en Leverziekten, Academisch Ziekenhuis, Leiden, The Netherlands.
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| pubmed:publicationType |
Journal Article
|