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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
14
|
| pubmed:dateCreated |
1989-6-30
|
| pubmed:databankReference | |
| pubmed:abstractText |
A rat kidney cDNA library was screened using an oligonucleotide hybridization probe derived from an amino acid sequence of the mouse erythrocyte Band 3 Cl-/HCO3- exchanger. Ten cDNAs representing a single class of mRNA and encoding the kidney Band 3 anion exchanger were isolated. Analysis of the deduced amino acid sequence revealed that kidney Band 3 is a truncated version of erythrocyte Band 3. The 848-amino acid rat kidney protein is virtually identical to mouse erythrocyte Band 3 except that it lacks the first 79 amino acids of the N-terminal cytoplasmic domain. Exons 1, 2, and 3 of the Band 3 gene, which are included in erythrocyte Band 3 mRNA, are not present in the kidney cDNA. The 5'-untranslated regions of kidney Band 3 transcripts contain sequences from the third intron and exons 4 and 5 of the Band 3 gene. The sequences from exons 4 and 5 that serve as untranslated sequences in the rat kidney mRNA have the potential to encode amino acids 46-79 of erythrocyte Band 3 but are not part of an open reading frame; the apparent translation initiation site corresponds to codon 80 of mouse erythrocyte Band 3 mRNA. Northern blot hybridization studies indicate that two additional Band 3 mRNAs, which differ in size from the major 4.5-kilobase pair transcript in kidney, are expressed at low levels in kidney and brain.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
264
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
8185-92
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:2722777-Amino Acid Sequence,
pubmed-meshheading:2722777-Animals,
pubmed-meshheading:2722777-Anion Exchange Protein 1, Erythrocyte,
pubmed-meshheading:2722777-Base Sequence,
pubmed-meshheading:2722777-DNA,
pubmed-meshheading:2722777-Erythrocytes,
pubmed-meshheading:2722777-Exons,
pubmed-meshheading:2722777-Humans,
pubmed-meshheading:2722777-Introns,
pubmed-meshheading:2722777-Kidney,
pubmed-meshheading:2722777-Mice,
pubmed-meshheading:2722777-Molecular Sequence Data,
pubmed-meshheading:2722777-Nucleic Acid Hybridization,
pubmed-meshheading:2722777-Oligonucleotide Probes,
pubmed-meshheading:2722777-Protein Biosynthesis,
pubmed-meshheading:2722777-RNA, Messenger,
pubmed-meshheading:2722777-Rats,
pubmed-meshheading:2722777-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2722777-Transcription, Genetic
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Primary structure of the rat kidney band 3 anion exchange protein deduced from a cDNA.
|
| pubmed:affiliation |
Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, Ohio 45267-0524.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|