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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
|
pubmed:dateCreated |
1989-7-13
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pubmed:abstractText |
Bombesin (BN) and BN-like peptides such as gastrin-releasing peptide (GRP) belong to the growing group of regulatory peptides. Bombesin and GRP share the same carboxyl-terminal heptapeptide sequence that is essential for receptor recognition and biologic activity. Immunohistochemical findings have indicated the presence of a BN-like peptide in the neuroendocrine cells of the human prostate. These findings, together with the high concentrations of different regulatory peptides recently found in seminal plasma, prompted us to characterize a BN-like peptide in human semen by gel-filtration chromatography, reverse-phase high-pressure liquid chromatography, and in vitro bioassay. Here we demonstrate that human semen contains high levels (mean +/- standard deviation, 288.9 +/- 114.39 pg/ml) of a substance that closely resembles the 14-27 carboxyl-terminal fragment of GRP (GRP 14-27). The partially purified peptide obtained from this source, examined on an in vitro bioassay that is especially useful for testing BN-like activities, elicits a contractile response of the rat uterus that is indistinguishable from that stimulated by standard BN and GRP 14-27. The GRP 14-27 present in human seminal plasma may be involved in some aspect of the process of fertilization.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
|
pubmed:issn |
0015-0282
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
51
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1034-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2721721-Adult,
pubmed-meshheading:2721721-Animals,
pubmed-meshheading:2721721-Bombesin,
pubmed-meshheading:2721721-Chromatography, Gel,
pubmed-meshheading:2721721-Chromatography, High Pressure Liquid,
pubmed-meshheading:2721721-Female,
pubmed-meshheading:2721721-Gastrin-Releasing Peptide,
pubmed-meshheading:2721721-Humans,
pubmed-meshheading:2721721-Male,
pubmed-meshheading:2721721-Peptides,
pubmed-meshheading:2721721-Radioimmunoassay,
pubmed-meshheading:2721721-Rats,
pubmed-meshheading:2721721-Reference Values,
pubmed-meshheading:2721721-Semen,
pubmed-meshheading:2721721-Uterine Contraction
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pubmed:year |
1989
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pubmed:articleTitle |
Isolation of a human seminal plasma peptide with bombesin-like activity.
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pubmed:affiliation |
Department of Andrology, University of Rome La Sapienza, Italy.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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