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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1989-7-10
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pubmed:abstractText |
The prevalent glyoxalase II (S-2-hydroxyacylglutathione hydrolase, EC 3.1.2.6, a form) of rat liver cytosol has been studied with a series of seven S-blocked glutathione derivatives. At pH 7.4 and 20 degrees C, only p-nitrobenzyl-S-glutathione was found completely inactive. All the other derivatives are linear competitive inhibitors of the enzyme. Ki values using S-D-lactoylglutathione as substrate are reported. Alkyl-S-glutathiones are weak inhibitors and their inhibition increases with the decrease of the length of the alkyl chain. The best inhibitors are those glutathione derivatives which contain a thioester bond (carbobenzoxy- and p-nitrocarbobenzoxy-S-glutathione) or a carbonyl group (p-chlorophenacyl-S-glutathione). Inhibition by carbobenzoxy-S-glutathione seems to be more complex since the double reciprocal plot shows deviation from linearity at low substrate concentration.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0013-9432
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
41
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
175-80
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2721483-Animals,
pubmed-meshheading:2721483-Cytosol,
pubmed-meshheading:2721483-Glutathione,
pubmed-meshheading:2721483-Isoenzymes,
pubmed-meshheading:2721483-Kinetics,
pubmed-meshheading:2721483-Liver,
pubmed-meshheading:2721483-Rats,
pubmed-meshheading:2721483-Structure-Activity Relationship,
pubmed-meshheading:2721483-Thiolester Hydrolases
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pubmed:year |
1989
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pubmed:articleTitle |
Effects of some S-blocked glutathione derivatives on the prevalent glyoxalase II (a form) of rat liver.
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pubmed:affiliation |
Department of Experimental Medicine, University of Perugia, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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