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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1989-6-12
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pubmed:abstractText |
Factor D depleted serum did not support hemolysis of rabbit erythrocytes and solubilization of performed immune complexes. Fluid phase C3 cleavage increased in a dose dependent manner when D protein was added to normal or to factor D depleted serum. During short incubation factor D concentrations were correlated with the capacity of serum to solubilize immune complexes and to lyse rabbit erythrocytes. With prolonged incubation, the hemolytic activity decreased in a factor D dose dependent manner. This was probably due to fluid phase breakdown of C3 and factor B in the presence of high factor D concentrations. Hypocomplementemic sera from patients with active systemic lupus erythematosus (SLE) did not support solubilization of bovine serum albumin (BSA) anti-BSA complexes when factor D was added in excess. Patients with polycystic kidney disease with reduced renal function and high factor D concentrations showed increased concentrations of circulating C3d/dg fragments. The possibility was considered that high factor D concentrations in uremia might promote fluid phase C3 degradation and thereby limit the in vivo efficiency of alternative pathway activation on target surfaces.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0165-2478
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
231-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2714846-Animals,
pubmed-meshheading:2714846-Antigen-Antibody Complex,
pubmed-meshheading:2714846-Complement Activation,
pubmed-meshheading:2714846-Complement C3,
pubmed-meshheading:2714846-Complement Pathway, Alternative,
pubmed-meshheading:2714846-Dose-Response Relationship, Drug,
pubmed-meshheading:2714846-Erythrocytes,
pubmed-meshheading:2714846-Hemolysis,
pubmed-meshheading:2714846-Humans,
pubmed-meshheading:2714846-Immunoelectrophoresis,
pubmed-meshheading:2714846-Lupus Erythematosus, Systemic,
pubmed-meshheading:2714846-Polycystic Kidney Diseases,
pubmed-meshheading:2714846-Rabbits,
pubmed-meshheading:2714846-Serine Endopeptidases
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pubmed:year |
1989
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pubmed:articleTitle |
Influence of factor D concentrations on fluid phase C3 activation, lysis of rabbit erythrocytes and solubilization of immune complexes.
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pubmed:affiliation |
Department of Rheumatology, University Hospital of Lund, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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