Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1989-6-19
pubmed:abstractText
Two methods are described for measuring the titres of melanin-concentrating hormone (MCH) in trout plasma. One involves the extraction of MCH from 1-ml plasma onto C18 Sep Pak cartridges, after which the eluted peptide is measured by conventional radioimmunoassay. In the alternative method, antibodies are bound onto immunobeads which are added to 0.5 ml plasma. After incubation for 24 hr, the beads are washed to remove the plasma and are incubated with 125I-labelled MCH; the following day, the labelled beads are separated by centrifugation, washed, and counted. The relative advantages of each method is discussed. Using these two methods, it is shown that the plasma concentration of the hormone is significantly higher in fish from white tanks (greater than 50 pmol/litre) than in fish from black tanks (approximately 10 pmol/litre) or those kept in the dark (approximately 5 pmol/litre). The plasma concentration of MCH changes rapidly when trout are moved from one coloured background to another, indicating its involvement in physiological colour change.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0016-6480
pubmed:author
pubmed:issnType
Print
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
221-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
The measurement of melanin-concentrating hormone in trout blood.
pubmed:affiliation
School of Biological Sciences, Bath University, England.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't