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rdf:type |
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lifeskim:mentions |
umls-concept:C0004793,
umls-concept:C0006556,
umls-concept:C0008266,
umls-concept:C0033684,
umls-concept:C0041080,
umls-concept:C0242949,
umls-concept:C0699788,
umls-concept:C1522642,
umls-concept:C1533691,
umls-concept:C1709634,
umls-concept:C1883254
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pubmed:issue |
1
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pubmed:dateCreated |
1989-6-22
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pubmed:databankReference |
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pubmed:abstractText |
The nucleotide sequence of several cDNA clones coding for the phosphate translocator from spinach chloroplasts has been determined. The cDNA clones were selected from a lambda gt10 library prepared from poly(A)+ mRNA of spinach leaves using oligonucleotide probes modeled from amino acid sequences of tryptic peptides prepared from the isolated translocator protein. A 1439 bp insert of one of the clones codes for the entire 404 amino acid residues of the precursor protein corresponding to a mol. wt of 44,234. The full-length clone includes 21 bp at the transcribed non-coding 5' region with the ribosome initiation sequence ACAATGG, a 1212 bp coding region and 199 bp at the non-coding 3' region excluding the poly(A) tail which starts 17 bp downstream from a putative polyadenylation signal, AATAAT. According to secondary structure predictions the mature part of the chloroplast phosphate translocator exhibits high hydrophobicity and consists of at least seven membrane-spanning segments. Using plasmid-programmed wheat germ lysate the precursor protein was synthesized in vitro and could be imported into spinach chloroplasts where it is inserted into the inner envelope membrane.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-16663686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-203230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-2822394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-2884042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-2953027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-2998756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-3000775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-3038521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-322279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-3291638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-3409889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-3474623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-3514209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-3526158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-3780721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-3882712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-3960719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-4009718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-4147558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-4502942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-6198242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-6235151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-6288471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-6329691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-648535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-6694911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-6731838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-6798029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-7099970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2714257-7108955
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
39-46
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:2714257-Amino Acid Sequence,
pubmed-meshheading:2714257-Base Sequence,
pubmed-meshheading:2714257-Chloroplast Proteins,
pubmed-meshheading:2714257-Chloroplasts,
pubmed-meshheading:2714257-DNA,
pubmed-meshheading:2714257-Membrane Proteins,
pubmed-meshheading:2714257-Membrane Transport Proteins,
pubmed-meshheading:2714257-Molecular Sequence Data,
pubmed-meshheading:2714257-Phosphates,
pubmed-meshheading:2714257-Plant Proteins,
pubmed-meshheading:2714257-Plants,
pubmed-meshheading:2714257-Protein Precursors,
pubmed-meshheading:2714257-Protein Processing, Post-Translational
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pubmed:year |
1989
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pubmed:articleTitle |
The triose phosphate-3-phosphoglycerate-phosphate translocator from spinach chloroplasts: nucleotide sequence of a full-length cDNA clone and import of the in vitro synthesized precursor protein into chloroplasts.
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pubmed:affiliation |
Institut für Biochemie der Pflanze, Göttingen, FRG.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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